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PFAM

Pfam is a comprehensive database of protein families and domains that classifies proteins based on sequence alignments and hidden Markov models, providing essential tools for protein annotation and evolutionary analysis.

Introduction

Understanding Pfam: The Gateway to Protein Family Analysis

Pfam stands as a fundamental resource in protein research, offering researchers detailed insights into protein families, domains, and evolutionary relationships through sophisticated computational approaches.

Core Components

  1. Database Content Primary Features:
  • Protein families
  • Domain definitions
  • Multiple alignments
  • Hidden Markov Models
  • Functional annotations
  • Evolutionary relationships
  1. Classification System Organization:
  • Pfam-A (curated)
  • Clans (related families)
  • Domain architectures
  • Family relationships
  • Sequential features
  • Structural mappings

Key Features

  1. Search Capabilities Search Options:
  • Sequence search
  • Domain architecture
  • Family browsing
  • Keyword search
  • Text mining
  • Structure mapping
  1. Analysis Tools Built-in Tools:
  • HMM scanning
  • Multiple alignment
  • Tree generation
  • Domain visualization
  • Architecture analysis
  • Sequence annotation

Research Applications

  1. Protein Analysis Primary Uses:
  • Domain identification
  • Function prediction
  • Evolutionary studies
  • Structure prediction
  • Annotation transfer
  • Family classification
  1. Comparative Genomics Applications:
  • Domain architecture
  • Family distribution
  • Evolutionary patterns
  • Functional annotation
  • Cross-species comparison

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