High throughput in situ imaging reveals complex ecological behaviour of giant marine mixotrophic protists

Mixotrophic organisms have the ability to combine photosynthetic and heterotrophic nutrition, and are ubiquitous in marine ecosystems. Here, we focus on Rhizaria, a diverse group of planktonic protists that included both mixotrophic and non-mixotrophic taxa, and that are particularly delicate and poorly sampled by standard plankton nets. Using high-frequency in situ imaging, we study the ecology of these organisms directly in their undisturbed environment. We examined the fine-scale distribution and orientation of ~230,000 organisms belonging to three groups of Rhizaria, including the mixotrophic taxa Acantharia and Collodaria, and the non-mixotrophic Phaeodaria. Overall, our results suggest