Global Nernstian astrocytic depolarization breaks down during local synaptic input

Substantial progress in glial electrophysiology has revealed that astrocytes, which account for half of the cells in the human brain, exhibit membrane potentials that often reflect changes in the extracellular environment. Such responses are mediated by a variety of biochemicals, including potassium and neurotransmitters. Recent advances in voltage imaging have provided new insights into voltage activity in astrocyte peripheries, revealing highly localized depolarization that depends on local presynaptic activity. However, the electrophysiological properties of these isolated peripherals have not been explored due to limitations of spatial and temporal resolution. In this study, we aimed to