Polarization-engineered aberration-resilient light sheet microscopy

Light sheet fluorescence microscopy enables volumetric imaging with high imaging speed, optical sectioning capability, and reduced photobleaching and phototoxicity, and has become a workhorse in bioimaging. However, widely adopted Gaussian light sheets face an inherent trade-off between axial resolution and field-of-view due to diffraction. State-of-the-art 'nondiffracting light sheets'--including Bessel beam, Airy beam, and lattice light sheet--alleviate this trade-off but suffer from optical aberrations that compromise performance with increasing imaging depth. While the integration of adaptive optics offers a promising solution, such integrated systems are typically complex, expensive, an