Establishment of Integration-Free iPSCs from Diverse Porcine Species: A Novel Resource for Conservation and African Swine Fever Research

The accelerating biodiversity crisis, compounded by emerging infectious diseases like African swine fever (ASF), necessitate innovative conservation and disease management. ASF susceptibility varies wildly across species, from near-100% mortality in Asian suids to asymptomatic carriage in African forest species. We report the first successful derivation of integration-free induced pluripotent stem cells (iPSCs) from four phylogenetically distinct species: wild boar (Sus scrofa), Bornean bearded pig (Sus barbatus), Babirusa (Babyrousa babyrussa), and Red river hog (Potamochoerus porcus). Using Sendai virus-mediated reprogramming, we achieved efficiencies between 0.003% and 0.26%. These iPSCs