ChIP-exo interrogation of Crp, DNA, and RNAP holoenzyme interactions

Numerous in vitro studies have yielded a refined picture of the structural and molecular associations between Cyclic-AMP receptor protein (Crp), the DNA motif, and RNA polymerase (RNAP) holoenzyme. In this study, high-resolution ChIP-exonuclease (ChIP-exo) was applied to study Crp binding in vivo and at genome-scale. Surprisingly, Crp was found to provide little to no protection of the DNA motif under activating conditions. Instead, Crp demonstrated binding patterns that closely resembled those generated by {sigma}70. The binding patterns of both Crp and {sigma}70 are indicative of RNAP holoenzyme DNA footprinting profiles associated with stages during transcription initiation that occur pos