Insertion patterns of P{lacW} and P{EP} artificial transposons on the third chromosome of Drosophila melanogaster

Insertional mutagenesis experiments performed on Drosophila melanogaster model often relies on induced mobilization of artificial transposons derived from P mobile element. In an attempt to detect transposition preferences, we accomplished a pilot study concerning the insertional patterns of P{lacW} and P{EP} constructs in the third chromosome of D. melanogaster. Our inventory inquiry considered 2177 insertions of P{lacW} and 1646 insertions of P{EP} available in FB2016_02 release of FlyBase and revealed insertional hotspots and coldspots in 3L and 3R, but also a preference of both artificial transposons to insert in 3R. The general distribution of P{lacW} and P{EP} insertions appears to be